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当前位置: 首页 > 产品中心 > Specialty_ELISA_kits > Gendepot/BrdU细胞增殖分析试剂盒,C0300/1000分析/C0300-100
商品详细Gendepot/BrdU细胞增殖分析试剂盒,C0300/1000分析/C0300-100
Gendepot/BrdU细胞增殖分析试剂盒,C0300/1000分析/C0300-100
Gendepot/BrdU细胞增殖分析试剂盒,C0300/1000分析/C0300-100
商品编号: C0300-100
品牌: Gen depot
市场价: ¥18700.00
美元价: 11220.00
产地: 美国(厂家直采)
公司:
产品分类: 特色ELISA试剂盒
公司分类: Specialty_ELISA_kits
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
ContentsBrdU Reagent,Fixing SolutionPrediluted Anti-BrdU DetectorPeroxidase Goat Anti-Mouse IgGConjugate DiluentSubstrate50X Plate Wash ConcwentrateStop Solution
ApplicationBrdU Cell Proliferation Assay

BrdU Proliferation Assay involves incorporation of BrdU into cells cultured in microtiter plates using the cell layer as the solid phase. During the final 2 to 24 hours of culture BrdU is added to wells of the microtiter plate. BrdU will be incorporated into the DNA of dividing cells. To enable antibody binding to the incorporated BrdU cells must be fixed, permeabilized and the DNA denatured. This is all done in one step by treatment with Fixing Solution. Detector anti-BrdU monoclonal antibody is pipetted into the wells and allowed to incubate for one hour, during which time it binds to any incorporated BrdU. Unbound antibody is washed away and horseradish peroxidase-conjugated goat anti-mouse antibody is added, which binds to the Detector Antibody.

The horseradish peroxidase catalyzes the conversion of the chromogenic substrate tetra-methylbenzidine (TMB) from a colorless solution to a blue solution (or yellow after the addition of stopping reagent), the intensity of which is proportional to the amount of incorporated BrdU in the cells. The colored reaction product is quantified using a spectrophotometer.

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