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当前位置: 首页 > 产品中心 > globulin > Gendepot/PureSelect蛋白G-琼脂糖快速流动,P9302/20ml/P9302-020
商品详细Gendepot/PureSelect蛋白G-琼脂糖快速流动,P9302/20ml/P9302-020
Gendepot/PureSelect蛋白G-琼脂糖快速流动,P9302/20ml/P9302-020
Gendepot/PureSelect蛋白G-琼脂糖快速流动,P9302/20ml/P9302-020
商品编号: P9302-020
品牌: Gen depot
市场价: ¥18200.00
美元价: 10920.00
产地: 美国(厂家直采)
公司:
产品分类: 球蛋白
公司分类: globulin
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
LigandRecombinant Streptococcal Protein G lacking the albumin-binding produced in E. coli
Binding capacity>20mg sheep IgG and 38mg human/ml
Resin Volume50% slurry
Bead Size Range90um (45-165um)
GenDEPOT Protein G-Agarose is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. The recombinant protein G ligand is coupled to 4% highly cross-linked agarose. The static bindingcapacity of Protein G-Agarose is greater than 20 mg sheep IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc. Protein G, a bacterial cell wall protein isolated from group G Streptococci, binds to mammalian IgGs mainly through Fc regions.Native protein G has 3 IgG binding domains and also sites for albumin and cell surface binding. The latter have been eliminated from recombinant protein G to reduce nonspecific binding. Although protein G has very similar tertiary structures to protein A, their amino acid compositions differ significantly, resultingin different binding characteristics. Protein G can be used for purification of mammalian monoclonal and polyclonal IgGs that do not bind well to protein A.
Protein G has greater affinity than protein A for most mammalian IgGs, especially for certain subclasses including human IgG3, mouse IgG1 and rat IgG2a. Unlike protein A, protein G does not bind to human IgM, IgD and IgA.
品牌介绍
Gendepot我们为您的自定义规模应用提供各种试剂,化合物,设备和树脂。我们的技术和定制的工作流程解决方案有助于扩展和补充生命科学工具提供商和应用市场的产品组合,例如分子诊断,疫苗生产,基于细胞的测定,大规模DNA / RNA纯化等。