4000-520-616
欢迎来到免疫在线!(蚂蚁淘生物旗下平台)  请登录 |  免费注册 |  询价篮
主营:主营:分子类,蛋白类,抗体类,生化类试剂
咨询热线电话
4000-520-616
当前位置: 首页 > 产品中心 > Reverse_transcriptase > Gendepot/M-MLV逆转录酶,M2410/10000单位/M2410-100
商品详细Gendepot/M-MLV逆转录酶,M2410/10000单位/M2410-100
Gendepot/M-MLV逆转录酶,M2410/10000单位/M2410-100
Gendepot/M-MLV逆转录酶,M2410/10000单位/M2410-100
商品编号: M2410-100
品牌: Gen depot
市场价: ¥1500.00
美元价: 900.00
产地: 美国(厂家直采)
公司:
产品分类: 反转录酶
公司分类: Reverse_transcriptase
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Unit DefinitionOne unit is defined as the amount of enzyme required to catalyze the incorporation of 1nmol of deoxyribonucleotide into acid-insoluble forms in 10 minutes at 37oC, using poly(A)-oligo(dT)12-18 as the template-primer. Standard cDNA Synthesis Conditions50mM Tris-HCl (pH8.3), 75mM KCl, 3mM MgCl2, 10mM DTT, 1.0mM each dATP, dGTP, dCTP, and dTTP, 0.2 mg radom hexamer,1-5mg RNA, 200units M-MLV RT. The reaction volume was 20ml and the incubation was 45 min at 42 C.
ApplicationFirst-strand cDNA synthesis.
Primer extension.
Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5kb). The enzyme is a product of the pol gene of M-MLV and consists of a single subunit with a molecular weight of 71kDa.
品牌介绍
Gendepot我们为您的自定义规模应用提供各种试剂,化合物,设备和树脂。我们的技术和定制的工作流程解决方案有助于扩展和补充生命科学工具提供商和应用市场的产品组合,例如分子诊断,疫苗生产,基于细胞的测定,大规模DNA / RNA纯化等。