4000-520-616
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商品详细Gendepot/抗DDK,4C5,单克隆,1ml,D4500/2x100ul/D4500-002
Gendepot/抗DDK,4C5,单克隆,1ml,D4500/2x100ul/D4500-002
Gendepot/抗DDK,4C5,单克隆,1ml,D4500/2x100ul/D4500-002
商品编号: D4500-002
品牌: Gen depot
市场价: ¥5200.00
美元价: 3120.00
产地: 美国(厂家直采)
公司:
产品分类: 单克隆抗体
公司分类: monoclonal_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the peptide epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the peptide tag.
Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered peptide, thus eliminating the need for antibodies to proteins from each newly cloned gene. Commonly used epitope tags include glutathione-S-transferase (GST), c-myc, 6-histidine (6X-His), FLAG, green fluorescent protein (GFP), maltose binding protein (MBP), influenza A virus haemagglutinin (HA), b-galactosidase, and GAL4. Anti-DDK,4C5 has been successfully used in Western blot and immunofluorescence procedures to detect the presence of fusion proteins containing the epitope tag sequence DYKDDDDK.
品牌介绍
Gendepot我们为您的自定义规模应用提供各种试剂,化合物,设备和树脂。我们的技术和定制的工作流程解决方案有助于扩展和补充生命科学工具提供商和应用市场的产品组合,例如分子诊断,疫苗生产,基于细胞的测定,大规模DNA / RNA纯化等。