Gendepot/CHIR 99021，GSK-3抑制剂，游离碱，BC212/2 mg/BC212-002
市场价：
¥1900.00
美元价：
1140.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| IC50 | 6.7 nM / 10 nM |
|---|---|
| Target | GSK-3β / GSK-3α |
| Solubility | 23.3mg/mL in DMSO |
CHIR 99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs CDC2 and ERK2, as well as other protein kinases. Furthermore, CHIR 99021 shows only weak binding to a panel of 22 pharmacologically relevant receptors and little inhibitory activity against a panel of 23 nonkinase enzymes. CHIR 99021 induces the activation of glycogen synthase (GS) in insulin receptor-expressing CHO-IR cells with EC50 of 0.763 μM.In addition to simulating the actions of insulin, inhibition of GSK-3 by CHIR 99021 (3 μM) increases free cytosolic β-catenin by 1.9-fold, mimicking the canonical Wnt signaling pathway in 3T3-L1 preadipocytes. During any of the first 3 days of differentiation, CHIR 99021 treatment inhibits the preadipocyte differentiation with IC50 of 0.3 μM by blocking induction of CCAAT/enhancer-binding protein α (C/EBPα) and peroxisome proliferator-activated receptor γ (PPARγ).Unlike lithium chloride and AR-A014418, CHIR-99021 treatment does not reduce the viability of INS-1E cells even at high concentrations. Instead, CHIR 99021 robustly increases the rate of proliferation of INS-1E cells in a dose-dependent manner, and significantly inhibits INS-E cell death induced by high glucose and high palmitate in a concentration-dependent manner. CHIR 99021 promotes primary beta cell replication in isolated rat islets at concentrations as low as 1 μM, with 2-3 fold increase of cell replication at 5 μM of CHIR 99021 treatment.
品牌介绍
Gendepot我们为您的自定义规模应用提供各种试剂，化合物，设备和树脂。我们的技术和定制的工作流程解决方案有助于扩展和补充生命科学工具提供商和应用市场的产品组合，例如分子诊断，疫苗生产，基于细胞的测定，大规模DNA / RNA纯化等。
品牌分类
生化试剂
Protein Biochemistry Reagent
蛋白质电泳试剂
抗体
Peptide
实验室必备耗材
Cell Based Assay Kit
Microbiology Reagent
生物分子
DNA Electrophoresis Reagent
IHC Kit and Reagent
蛋白质分离纯化
植物培养
Cell/Tissue Culture
Cloning Reagent
Lab Essential Equipment
Cytokines
分析试剂盒
Biological Buffers
Growth Factor
IP & Reagent
分子生物学试剂
DNA/RNA纯化试剂
Enzymes
ELISA试剂盒和试剂
PCR/RT-PCR Reagent
Protein Assay Reagent
Immunochemical Reagent
蛋白质印迹试剂
转染试剂
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