Gendepot/环胺，>99%，BC019/10 mg/BC019-010
市场价：
¥2160.00
美元价：
1296.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| IC50 | 46 nM in TM3Hh12 cells. |
|---|---|
| Biochem/physiol Actions | Cyclopamine is a Hedgehog signaling pathway inhibitor; inhibits the growth of medulloblastoma cells. |
| Solubility | 10 mg/mL warmed (24.29 mM) in DMF2 mg/mL warmed (4.85 mM) in Ethanol |
Cyclopamine inhibits the Hedgehog signaling pathway with an IC50 of 46 nM, and blocks the activity of human Smo receptor expressed in CHO-K1 cells in [3H]Hh-Ag binding assay with an IC50 of 280 nM.Cyclopamine significantly inhibits Hedgehog pathway activity in a dose-dependent manner in gut-derived tumor cell lines expressing Patched (PTCH) mRNA, and induces growth inhibition of those tumor cell lines by 75-95% at the concentration of 3 μM, but ineffective towards the colon tumor cells without PTCH mRNA expression, suggesting the effects of Cyclopamine treatment are Hedgehog pathway related rather than generally cytotoxic.By blocking Hedgehog signaling through direct interaction with Smo, Cyclopamine (10 μM) inhibits the proliferation of SMOhighCyclopamine-responsive cell lines L3.6sl and Panc 05.04 by 75-80%, and increases the apoptosis by 2.5- to 3.5-fold, without affecting the BxPC3-SMOlowcell line.Cyclopamine treatment significantly decreases of Snail mRNA and increasea E-cadherin transcripts in the E3LZ10.7 cell line. Independent of inhibition of cell growth, Cyclopamine treatment significantly inhibits the invasive phenotype of Hedgehog-dependent L3.6pl cells, causing a >500-fold reduction in the number of transmigrating cells, but not that of the Hedgehog-independent cell line Panc-1.
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Gendepot我们为您的自定义规模应用提供各种试剂，化合物，设备和树脂。我们的技术和定制的工作流程解决方案有助于扩展和补充生命科学工具提供商和应用市场的产品组合，例如分子诊断，疫苗生产，基于细胞的测定，大规模DNA / RNA纯化等。
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生化试剂
Protein Biochemistry Reagent
蛋白质电泳试剂
抗体
Peptide
实验室必备耗材
Cell Based Assay Kit
Microbiology Reagent
生物分子
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Lab Essential Equipment
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分析试剂盒
Biological Buffers
Growth Factor
IP & Reagent
分子生物学试剂
DNA/RNA纯化试剂
Enzymes
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PCR/RT-PCR Reagent
Protein Assay Reagent
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蛋白质印迹试剂
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