Gendepot/Hayflick培养基,含氨苄西林,H2486/100ml/H2486-100
市场价:
¥2700.00
美元价:
1620.00
产品分类:
培养基
公司分类:
culture_medium
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Mycoplasma, represents a group of minute bacteria devoid of cell walls belonging to class Mollicutes. These are responsible for causing serious contamination in cell and or tissue cultures used to generate compendial articles. They may also cause contamination of filtered sterlized Soybean Casein Digest Broth. Infection of cells in a culture can affect nearly every pathway of cell metabolism including alteration of the cells phenotypical characterization and normal growth. The presence of mycoplasma species does not always result in turbid growth in cultures or visible alteration of the cells. Hayflick et al have reported complex medias for growth of Mycoplasmas.
Testing of mycoplasmas is necessary to assure reliably pure biotech products and allied materials used to generate these products. Hayflick Medium Complete is recommended for general detection of Mycoplasmas in Pharmacopoeias for testing of products for Mycoplasma. This medium contains Beef heart infusion broth containing beef heart infusion and peptone which provides nitrogen, vitamins, amino acids, and carbon sources. Sodium chloride maintains the osmotic balaces. Many Mycoplasmas require serum for their good growth. Addition of Ampicillin surpress growth of unwanted flora.
Added Horse serum provides growth factors including liqid components to My coplasma. DNA provides growth factors including lipid components to Mycoplasma. Yeast extract serves rich source of Nicotinamide-Adenine Dinucleotide (NAD) required by M.synoviae. Mycoplasma species are either aerobic or facultative anaerobic but some are microaerophilic. Few are anaerobic saprophytic Mycoplasma which grow best at 22-35oC while pathogenic strains grow at 35oC.
Tubes should be incubated in an atmosphere containing 5-10% carbon dioxide and examined after incubation of 48 hours but they should not be discarded as negative until after incubation for three weeks. M.synoviae is not able to grow on Hayflick broth medium be cause growth depends on NAD.
品牌介绍
Gendepot我们为您的自定义规模应用提供各种试剂,化合物,设备和树脂。我们的技术和定制的工作流程解决方案有助于扩展和补充生命科学工具提供商和应用市场的产品组合,例如分子诊断,疫苗生产,基于细胞的测定,大规模DNA / RNA纯化等。
品牌分类
生化试剂
Protein Biochemistry Reagent
蛋白质电泳试剂
抗体
Peptide
实验室必备耗材
Cell Based Assay Kit
Microbiology Reagent
生物分子
DNA Electrophoresis Reagent
IHC Kit and Reagent
蛋白质分离纯化
植物培养
Cell/Tissue Culture
Cloning Reagent
Lab Essential Equipment
Cytokines
分析试剂盒
Biological Buffers
Growth Factor
IP & Reagent
分子生物学试剂
DNA/RNA纯化试剂
Enzymes
ELISA试剂盒和试剂
PCR/RT-PCR Reagent
Protein Assay Reagent
Immunochemical Reagent
蛋白质印迹试剂
转染试剂
显示分类
联络我们