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当前位置: 首页 > 产品中心 > Other_buffers > Gendepot/Laemmli样品缓冲液(4X),还原,L1100/10 ml/L1100-001
商品详细Gendepot/Laemmli样品缓冲液(4X),还原,L1100/10 ml/L1100-001
Gendepot/Laemmli样品缓冲液(4X),还原,L1100/10 ml/L1100-001
Gendepot/Laemmli样品缓冲液(4X),还原,L1100/10 ml/L1100-001
商品编号: L1100-001
品牌: Gen depot
市场价: ¥500.00
美元价: 300.00
产地: 美国(厂家直采)
公司:
产品分类: 其它缓冲液
公司分类: Other_buffers
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Concentration(4X)Tris-Hcl(pH 6.8) : 250 mMSDS : 8 %Glycerol : 40 %2-Mercaptoethanol : 8 %Bromophenol blue : 0.02%
ApplicationProtein Electrophoresis
Laemmli sample buffer is especially formulated for protein sample preparation to be used in the Laemmli SDS-PAGE system. A protein sample is mixed with the 4X sample buffer and heated in boiling water for 5-10 min. The 2-mercaptoethanol reduces the intra and inter-molecular disulfide bonds. The SDS detergent denatures the proteins and subunits and gives each an overall negative charge so that each will separate based on size. The bromophenol blue serves as a dye front that runs ahead of the proteins and also serves to make it easier to see the sample during loading. The glycerol increases the density of the sample so that it will layer in the sample well.
品牌介绍
Gendepot我们为您的自定义规模应用提供各种试剂,化合物,设备和树脂。我们的技术和定制的工作流程解决方案有助于扩展和补充生命科学工具提供商和应用市场的产品组合,例如分子诊断,疫苗生产,基于细胞的测定,大规模DNA / RNA纯化等。