Downstream purification is the leading disadvantage to using serum in cell culture media. For example, monoclonal antibody production and recombinant protein secretion both require purification to remove serum-derived gamma globulin from the media.
Insulin-Transferrin-Selenium Solution II(100X) , is a growth supplement that should be added at 10 mL/L medium. Supple-mentation enables a reduction in the FBS requirements of the culture. The components of ITS are required for optimal perfor-mance of cells in serum-free culture. Some other trace elements and nutrients are contained additionally.
All components are derived from non-animal source
Components
Recombinant HAS : 0.05g/LRecombinant Insulin ( 28 units/mg) : 0.5g/LRecombinant Transferrin : 0.6g/LSodium Selenite : 0.00067g/LEthanolamine : 0.01g/LCuSO4 : 0.0000016g/LZnSO4 : 0.000863g/LFerric citrate : 0.00204g/LChemically Defined Nutrients : 0.16g/L
